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Phosphorylation of Thr695 and Thr850 on the myosin phosphatase target subunit: inhibitory effects and occurrence in A7r5 cells.
Murányi, Andrea; Derkach, Dmitry; Erdodi, Ferenc; Kiss, Andrea; Ito, Masaaki; Hartshorne, David J.
Afiliação
  • Murányi A; Muscle Biology Group, Department of Nutritional Sciences, University of Arizona, 1177 E. 4th Street, Shantz 627, Tucson, AZ 85721-0038, USA.
FEBS Lett ; 579(29): 6611-5, 2005 Dec 05.
Article em En | MEDLINE | ID: mdl-16297917
Major sites for Rho-kinase on the myosin phosphatase target subunit (MYPT1) are Thr695 and Thr850. Phosphorylation of Thr695 inhibits phosphatase activity but the role of phosphorylation at Thr850 is not clear and is evaluated here. Phosphorylation of both Thr695 and Thr850 by Rho-kinase inhibited activity of the type 1 phosphatase catalytic subunit. Rates of phosphorylation of the two sites were similar and efficacy of inhibition following phosphorylation was equivalent for each site. Phosphorylation of each site on MYPT1 was detected in A7r5 cells, but Thr850 was preferred by Rho-kinase and Thr695 was phosphorylated by an unidentified kinase(s).
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Treonina / Fosfatase de Miosina-de-Cadeia-Leve Limite: Animals Idioma: En Revista: FEBS Lett Ano de publicação: 2005 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Treonina / Fosfatase de Miosina-de-Cadeia-Leve Limite: Animals Idioma: En Revista: FEBS Lett Ano de publicação: 2005 Tipo de documento: Article