Truncated APC is required for cell proliferation and DNA replication.
Int J Cancer
; 119(1): 74-9, 2006 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-16450383
ABSTRACT
The tumour suppressor APC is truncated in most colon cancers, which leads to the stabilization of beta-catenin and to the constitutive activation of Wnt signalling. However, it is not clear why colon cancer cells retain the truncated APC fragment. Here, we show that a decrease of APC levels achieved by RNA interference impairs cell proliferation and DNA replication, not only in 293 cells that express a wild-type protein, but also in SW480 colon cancer cells that express exclusively a truncated APC fragment. This correlates with a reduction of the levels of cyclin A, cyclin A-dependent kinase activity, p27(kip1) and the catalytic subunit of DNA polymerase delta. Thus, our data suggest that colon cancer cells retain a truncated APC fragment because it is essential for cell proliferation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes APC
/
Neoplasias do Colo
/
Proliferação de Células
/
Replicação do DNA
Limite:
Humans
Idioma:
En
Revista:
Int J Cancer
Ano de publicação:
2006
Tipo de documento:
Article