Cultured human keratinocytes cannot metabolize vitamin D3 to 25-hydroxyvitamin D3.
FEBS Lett
; 282(2): 409-11, 1991 May 06.
Article
em En
| MEDLINE
| ID: mdl-1645286
ABSTRACT
The metabolism of [3H]vitamin D3 was studied in cultured human keratinocytes (CHK). Intact CHK were incubated for 1, 6, 12, 24 and 48 h with [3H]vitamin D3 and the lipid soluble fractions from the media and cells were extracted by high-performance liquid chromatography (HPLC). Vitamin D3 and its metabolites, 25-OH-D3, 24,25(OH)2D3 and 1,25(OH)2D3 were added to the extracts, as markers, prior to HPLC. HPLC analysis of the lipid extracts did not reveal any monohydroxylated metabolites. CHK incubated for one hour with [3H]25-OH-D3 showed a 10 +/- 4% conversion to [3H]1,25(OH)2D3 whereas no conversion to [3H]1,25(OH)2D3 was observed in control CHKs that were boiled prior to incubation with [3H]25-OH-D3. These findings suggest that cultured neonatal keratinocytes are incapable of metabolizing vitamin D3 to 25-OH-D3.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Calcifediol
/
Queratinócitos
/
Colecalciferol
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1991
Tipo de documento:
Article