Novel apoptosis assay to analyze immunosuppression.
Cytometry A
; 69(3): 158-60, 2006 Mar.
Article
em En
| MEDLINE
| ID: mdl-16479615
BACKGROUND: We developed a novel whole blood assay to assess the effects of immunosuppressants on lymphocyte apoptosis. METHODS: Peripheral blood for six experiments for each drug was treated either with cyclosporin A (CsA), mycophenolate acid (MPA), tacrolimus (TRL) or rapamycin (RAPA). Whole blood was stimulated with different concentrations of staurosporine (0-5 microM) for 24 h. Using FACS, apoptosis were measured by Annexin V expression (%cells +/- SEM). RESULTS: MPA, RAPA, TRL, but not CsA, increased (P < 0.05) apoptotic cells (MPA:20.9 +/- 3.7; RAPA:17.5 +/- 3.3; TRL:16.4 +/- 2.9 vs. control:15.2 +/- 2.8). CONCLUSION: This new whole blood assay exhibited that MPA has a stronger effect on apoptosis than RAPA and TRL, whereas CsA had no effect.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Terapia de Imunossupressão
/
Apoptose
/
Imunossupressores
Limite:
Humans
Idioma:
En
Revista:
Cytometry A
Ano de publicação:
2006
Tipo de documento:
Article