[Expression changes of interleukin-1 receptor associated kinase-4 during endotoxin tolerance development in kupffer cells].
Zhonghua Gan Zang Bing Za Zhi
; 14(2): 97-100, 2006 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-16494776
ABSTRACT
OBJECTIVE:
To explore the mechanism of endotoxin tolerance (ET) through observing the expression of interleukin 1 receptor associated kinase-4 (IRAK-4) during endotoxin tolerance development in Kupffer cells (KCs).METHODS:
Isolated KCs of Balb/c mouse were divided into two groups the non-endotoxin tolerance (NET) group and the endotoxin tolerance (ET) group, which were pretreated with 10 ng/ml lipopolysaccharide (LPS) for 24 h. Then, the two groups were treated with 100 ng/ml LPS. The expressions of IRAK-4 gene and protein level were determined by RT-PCR and Western blot. The activities of NF-kappaB of KCs and the TNFalpha level were estimated by ELISA at 0 h, 1 h, 3 h, 6 h and 12 h after LPS stimulation.RESULTS:
The ultimate level of IRAK-4, the activities of NF-kappaB and the TNFalpha level were evidently lower in the ET group than those in the NET group (t = 12.4, 17.4 and 138.9 respectively, P<0.01).CONCLUSIONS:
Pretreatment with LPS on KCs could induce endotoxin tolerance of KCs and inhibition of IRAK-4 expression may be one of the reasons for its development.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endotoxinas
/
Quinases Associadas a Receptores de Interleucina-1
/
Tolerância Imunológica
/
Células de Kupffer
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Ano de publicação:
2006
Tipo de documento:
Article