Transgene insertion pattern analysis using genomic walking in a transgenic mouse line.
Exp Anim
; 55(1): 65-9, 2006 Jan.
Article
em En
| MEDLINE
| ID: mdl-16508214
ABSTRACT
A transgene mapping technique (Noguchi et al., Exp. Anim. 53103-111, 2004) is described that can be used to analyze transgene integration patterns in transgenic mice. The technique was used to reveal that a transgenic mouse line (GM1-sy#116) harbored inverted and direct tandem repeats of both intact and partial pCAGGS-based transgenes in the G2 region of chromosome 1. This complicated concatenation of transgenes may have been caused by simple end-joining of DNA constructs fragmented by exposure to UV transillumination during gel-purification, and by nuclease digestion inside zygote pronuclei. The results suggest that care should be taken to avoid unwanted fragmentation during the preparation of vector constructs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Camundongos Transgênicos
/
Passeio de Cromossomo
/
Transgenes
Limite:
Animals
Idioma:
En
Revista:
Exp Anim
Ano de publicação:
2006
Tipo de documento:
Article