Dopamine plasma membrane transporter (DAT) in rat thymus and spleen: an immunochemical and immunohistochemical study.
Auton Autacoid Pharmacol
; 26(2): 183-9, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16553646
ABSTRACT
The expression of the dopamine plasma membrane transporter (DAT) was investigated in rat thymus and spleen by immunochemical and immunohistochemical techniques. Antibodies raised against a peptide mapping near the amino terminus of DAT were bound to a single band of approximately 76 kDa in thymus and spleen membranes as well as in striatal and kidney membranes which were used as dopaminergic reference tissues. Reverse transcription-polymerase chain reaction analysis revealed that both thymus and spleen expressed DAT mRNA. Immunohistochemistry revealed in rat thymus a DAT immune reaction in the wall of arteries located in septa of connective tissue as well as in the medulla, with a reticular localization and an apparent negative reaction of thymocytes. In the spleen, DAT immunoreactivity was located primarily in the red-white pulp marginal zone, within small cells, likely corresponding to lymphocytes and in the wall of white pulp arteries. The presence of a dopamine transporter suggests that dopamine released in the lymphoid microenvironment may contribute to neuroimmune modulation. It cannot be excluded a different activity of dopamine in primary and secondary immune organs, such as maturation and selection of lymphocytes and activation of immune responses in the spleen.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Baço
/
Timo
/
Proteínas da Membrana Plasmática de Transporte de Dopamina
Limite:
Animals
Idioma:
En
Revista:
Auton Autacoid Pharmacol
Ano de publicação:
2006
Tipo de documento:
Article