GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells.
Mol Immunol
; 44(4): 521-9, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-16574228
ABSTRACT
RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lalpha, IL-lbeta, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFbeta, TNFalpha and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Transdução de Sinais
/
Citocinas
/
Receptores de Fator Estimulador de Colônias de Granulócitos
/
Apresentação de Antígeno
/
Interferência de RNA
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mol Immunol
Ano de publicação:
2007
Tipo de documento:
Article