Analysis of the thermostability determinants of hyperthermophilic esterase EstE1 based on its predicted three-dimensional structure.

Rhee, Jin-Kyu; Kim, Do-Yun; Ahn, Dae-Gyun; Yun, Jung-Hyuk; Jang, Seung-Hwan; Shin, Hang-Cheol; Cho, Hyun-Soo; Pan, Jae-Gu; Oh, Jong-Won

Rhee, Jin-Kyu; Kim, Do-Yun; Ahn, Dae-Gyun; Yun, Jung-Hyuk; Jang, Seung-Hwan; Shin, Hang-Cheol; Cho, Hyun-Soo; Pan, Jae-Gu; Oh, Jong-Won.

Afiliação

Rhee JK; Department of Biotechnology, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, South Korea.

Appl Environ Microbiol ; 72(4): 3021-5, 2006 Apr.

Article em En | MEDLINE | ID: mdl-16598011

ABSTRACT

ABSTRACT

The three-dimensional (3D) structure of the hyperthermophilic esterase EstE1 was constructed by homology modeling using Archaeoglobus fulgidus esterase as a reference, and the thermostability-structure relationship was analyzed. Our results verified the predicted 3D structure of EstE1 and identified the ion pair networks and hydrophobic interactions that are critical determinants for the thermostability of EstE1.

Assuntos

Archaeoglobus fulgidus/enzimologia; Esterases/química; Esterases/metabolismo; Temperatura Alta; Estabilidade Enzimática; Interações Hidrofóbicas e Hidrofílicas; Modelos Moleculares; Dados de Sequência Molecular; Desnaturação Proteica; Estrutura Secundária de Proteína; Alinhamento de Sequência; Relação Estrutura-Atividade

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Archaeoglobus fulgidus / Esterases / Temperatura Alta Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Appl Environ Microbiol Ano de publicação: 2006 Tipo de documento: Article

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