Comparison of preparation techniques of mixed samples (fungi-helminth eggs) for scanning electron microscopy by critical point drying.
Parasitol Res
; 99(4): 455-8, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-16607559
ABSTRACT
We compared three preparation techniques for critical point drying of fungus Paecilomyces lilacinus (Thom) Samson with Toxocara canis (Werner) Johnston and Taenia hydatigena Linneo eggs by scanning electron microscopy. We evaluated filtration (first), centrifugation (second), and phytoplankton network (third) in critical point drying methods. The first and third methods were advantageous for T. canis eggs because they preserved the quantity and quality of samples to obtain better images definition. The best technique for T. hydatigena eggs was the addition of phytoplankton network in critical point drying which preserved these helminth eggs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óvulo
/
Taenia
/
Microscopia Eletrônica de Varredura
/
Toxocara canis
/
Liofilização
/
Fungos
Limite:
Animals
Idioma:
En
Revista:
Parasitol Res
Ano de publicação:
2006
Tipo de documento:
Article