Technical note: a monoclonal antibody-based immunoassay for determination of ractopamine in swine feeds.
J Anim Sci
; 84(5): 1248-51, 2006 May.
Article
em En
| MEDLINE
| ID: mdl-16612028
ABSTRACT
An ELISA was developed for routine screening of ractopamine in swine feeds. Swine feed samples were extracted and purified, and the aqueous portion of the extract was analyzed for ractopamine using ELISA and confirmed by HPLC. For swine complex feeds containing ractopamine at 2.5 to 40 mg/kg, the average recoveries ranged from 73.1 to 86.5% by ELISA and 81.9 to 98.2% by HPLC. For the swine supplement containing ractopamine at 50 to 400 mg/kg, the average recoveries were 105.5 to 111.4% by ELISA and 89.1 to 92.9% by HPLC. The limit of detection was 0.24 microg/g by ELISA and 0.48 microg/g by HPLC, respectively. Results from the swine complex feeds (P = 0.009) and the supplement (P = 0.005) using ELISA and HPLC were not highly correlated. The ELISA was more sensitive and rapid and less expensive than the HPLC method and could be used for ractopamine screening in swine feeds before confirmation and quantification by other methods, such as HPLC.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenetilaminas
/
Suínos
/
Ensaio de Imunoadsorção Enzimática
/
Ração Animal
/
Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Revista:
J Anim Sci
Ano de publicação:
2006
Tipo de documento:
Article