Immunohistochemical and serological 90K/Mac-2BP detection in hepatocellular carcinoma patients: different behaviour of two monoclonal antibodies.
Med Chem
; 1(2): 185-9, 2005 Mar.
Article
em En
| MEDLINE
| ID: mdl-16787313
ABSTRACT
To clarify the biological role of the 90K/Mac-2BP glycoprotein, we evaluated the ability of two MAbs SP-2 and 1A4.22, to reveal this glycoprotein in both serum and tissue from hepatocellular carcinoma (HCC) patients. Tissue expression of 90K was detected by the immunohistochemical method in 20 HCC patients, while the 90K serum level was assessed by the ELISA assay in 13 HCC patients. MAb SP-2 was reactive only in serum, with a mean value of 12.8+/- 6.7 microg/ml . On the contrary, MAb 1A4.22 revealed immunoreactivity both in 92% of sera and in 60% of neoplastic samples. Positive staining was seen only in the epithelial cells and was cytoplasmic and granular in all instances. The mean 90K serum level assayed with MAb 1A4.22 was 29.4 +/- 13.7 microg/ml. Patients with a 90K serum level
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicoproteínas de Membrana
/
Carcinoma Hepatocelular
/
Células Epiteliais
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Anticorpos Monoclonais
/
Especificidade de Anticorpos
/
Antígenos de Neoplasias
Tipo de estudo:
Diagnostic_studies
Limite:
Aged
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Female
/
Humans
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Male
Idioma:
En
Revista:
Med Chem
Ano de publicação:
2005
Tipo de documento:
Article