Synthetic nucleosides and nucleotides. 43. Inhibition of vertebrate telomerases by carbocyclic oxetanocin g (C.OXT-G) triphosphate analogues and influence of C.OXT-G treatment on telomere length in human HL60 cells.
Nucleosides Nucleotides Nucleic Acids
; 25(4-6): 539-51, 2006.
Article
em En
| MEDLINE
| ID: mdl-16838844
ABSTRACT
Telomerase, responsible for telomere synthesis, is expressed in approximately 90% of human tumor cells but seldom in normal somatic cells. In this study, inhibition by carbocyclic oxetanocin G triphosphate (C. OXT-GTP) and its analogues was investigated in order to clarify the susceptibility of telomerase to various nucleotide analogues. C. OXT-GTP competitively inhibited telomerase activity with respect to dGTP However, C. OXT-GTP had a potent inhibitory effect on DNA polymerase alpha. It was examined whether the nucleoside (C. OXT-G) was able to alter telomere length in cultured human HL60 cells. Contrary to expectation, long-term treatment with 10 microM C. OXT-G was found to cause telomere lengthening.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Telômero
/
Telomerase
/
Guanina
/
Guanosina Trifosfato
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nucleosides Nucleotides Nucleic Acids
Ano de publicação:
2006
Tipo de documento:
Article