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Analysis of hormonal steroids in fish plasma and bile by coupling solid-phase extraction to GC/MS.
Budzinski, H; Devier, M H; Labadie, P; Togola, A.
Afiliação
  • Budzinski H; Laboratoire de Physico- et Toxico-Chimie (LPTC), UMR 5472 CNRS, Université Bordeaux I, 351 cours de la libération, 33405 Talence Cedex, France. h.budzinski@lptc.u-bordeaux1.fr
Anal Bioanal Chem ; 386(5): 1429-39, 2006 Nov.
Article em En | MEDLINE | ID: mdl-16906384
An analytical procedure for the simultaneous determination of twelve endogenous steroids (testosterone, androstenedione, 17beta-estradiol, estrone, pregnenolone, progesterone, dihydroandrostenedione, dihydrotestosterone, 11alpha-ketotestosterone, 17alpha-hydroxyprogesterone, 17alpha-hydroxypregnenolone, 17alpha,20beta-dihydroxy-4-pregnen-3-one) in plasma and bile samples by solid-phase extraction (SPE) and gas chromatography/mass spectrometry (GC-MS) has been developed. After enzymatic hydrolysis for bile samples only, samples were concentrated and purified using two successive SPE (C(18) and NH(2)) cartridges. Analytes were derivatized with a mixture of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) / mercaptoethanol / ammonium iodide (NH(4)I) and determined by GC-MS in selective ion monitoring mode. For most of the steroids monitored, recoveries were in the range 90-120% in plasma and in the range 60-70% in bile, and the reproducibility was below 10% for the complete procedure. Limits of detection obtained ranged from 0.1 to 0.4 ng/g in fish plasma and from 1.6 to 14 ng/g in fish bile. The developed method was successfully applied to the determination of plasma steroids in flounders (Platichthys flesus) collected from two French estuaries.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasma / Esteroides / Bile / Microextração em Fase Sólida / Cromatografia Gasosa-Espectrometria de Massas Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2006 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasma / Esteroides / Bile / Microextração em Fase Sólida / Cromatografia Gasosa-Espectrometria de Massas Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2006 Tipo de documento: Article