[The dynamics of microorganism populations and fermentation characters of co-cultures of rumen fungi and cellulolytic bacteria on different substrates].
Wei Sheng Wu Xue Bao
; 46(3): 422-6, 2006 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-16933613
ABSTRACT
In vitro co-culture technique and DGGE were used to investigate the dynamics of microorganism populations and fermentation characters of co-cultures of rumen fungi and cellulolytic bacteria at different substrates (concentrate to crude ratio in treatment A to E was all rice straw, 37, 55, 73 and all corn, respectively). The results showed that, compared with Oh, fungal population density at 24h increased in treatment B and C, but decreased in treatment A and D, and no fungi was detected in treatment E; but cellulolytic bacteria population at 24h increased with the increasing of concentrate to crude ratio. At the end of 48h fermentation, no fungi were detected in all treatments; cellulolytic bacteria population increased from treatment A to treatment C, but decreased from treatment D. DGGE results showed that samples collected in treatment A, B and C had similar DGGE patterns with about 11 dominant bands, but dominant bands in treatment D and E decreased markedly compared to treatments A, B and C. With the increasing of concentrate to crude ratio, pH value of the co-culture decreased dramatically (P < 0.05). During the fermentation periods, acetate was the major VFA in co-culture, the acetate to propionate ratio decreased from treatment A to treatment C, but increased from treatment D. With the increasing of concentrate to crude ratio, the total VFA at 48h increased from treatment A to treatment C, and then decreased. At the end of 48h fermentation, CMCase activity and xylanase activity were highest in treatment A. alpha-Amylase activity increased from treatment A to treatment D, but treatment E was the lowest in all treatments.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rúmen
/
Bactérias
/
Celulose
/
Fermentação
/
Fungos
Limite:
Animals
Idioma:
Zh
Revista:
Wei Sheng Wu Xue Bao
Ano de publicação:
2006
Tipo de documento:
Article