The promoter of the proliferating cell nuclear antigen (PCNA) gene is active in serum-derived cells.
Biochem Biophys Res Commun
; 169(2): 509-16, 1990 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-1694073
ABSTRACT
mRNA levels for the Proliferating Cell Nuclear Antigen (PCNA) gene are growth regulated. PCNA promoters of different lengths were used to drive a linked reporter, the cDNA for human thymidine kinase (TK). After transfection into TK ts13 cells, stable cell lines were obtained. Regardless of promoter length, in all cell lines the levels of TK mRNA were roughly similar in serum-deprived and serum stimulated cells, confirming, by an independent method, that the growth regulation of PCNA mRNA levels doe not depend on the 5' flanking sequence of the PCNA gene.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Regiões Promotoras Genéticas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1990
Tipo de documento:
Article