[Preparation and identification of rabbit antibody against LRR of Nogo-66 receptor (NgR)].

ABSTRACT

AIM: To prepare rabbit antibody against LRR of Nogo-66 receptor (NgR) and identify its properties. METHODS: LRR protein was expressed in E.coli BL21. Rabbits were immunized with purified LRR protein to prepare anti-LRR antibody. The titer and specificity of prepared antibodies were identified by Western blot and immunohistochemical staining, respectively. RESULTS: Purified rabbit anti-rat LRR antibody with high titer was obtained. The anti-LRR Ab showed good specificity and could be used to detect the NgR in rat brain and spinal cord tissues. Immunohistochemical staining indicated that NgR was expressed widely in spinal cord neurons. CONCLUSION: Successful preparation of anti-rat LRR antibody provides a useful tool for identification and further functional study of NgR molecule.