[Preparation and identification of rabbit antibody against LRR of Nogo-66 receptor (NgR)].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 22(5): 633-5, 2006 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-16948913
ABSTRACT
AIM:
To prepare rabbit antibody against LRR of Nogo-66 receptor (NgR) and identify its properties.METHODS:
LRR protein was expressed in E.coli BL21. Rabbits were immunized with purified LRR protein to prepare anti-LRR antibody. The titer and specificity of prepared antibodies were identified by Western blot and immunohistochemical staining, respectively.RESULTS:
Purified rabbit anti-rat LRR antibody with high titer was obtained. The anti-LRR Ab showed good specificity and could be used to detect the NgR in rat brain and spinal cord tissues. Immunohistochemical staining indicated that NgR was expressed widely in spinal cord neurons.CONCLUSION:
Successful preparation of anti-rat LRR antibody provides a useful tool for identification and further functional study of NgR molecule.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Superfície Celular
/
Sequências Repetitivas de Aminoácidos
/
Anticorpos
/
Proteínas da Mielina
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Ano de publicação:
2006
Tipo de documento:
Article