SWAP-70 associates transiently with macropinosomes.
Eur J Cell Biol
; 86(1): 13-24, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17046101
ABSTRACT
Cells accomplish the non-selective uptake of extracellular fluids, antigens and pathogens by the endocytic process of macropinocytosis. The protein SWAP-70 is a widely expressed, pleckstrin-homology (PH) domain-containing protein that marks a transitional subset of actin filaments in motile cells. Here we report that the protein SWAP-70 associates transiently with macropinosomes in dendritic cells and NIH/3T3 fibroblasts. The association of SWAP-70 with macropinosomes is preceded by the accumulation of Rac-GTP and followed by that of Rab5. Three regions of SWAP-70, the N-terminal region, the PH domain and the C-terminal region, contribute in a combinatorial manner to the transient association with newly formed macropinosomes in the cell periphery and occasionally with aged macropinosomes on their passage to the cell center. These data identify SWAP-70 as a transient component of early macropinosomes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pinocitose
/
Proteínas Nucleares
/
Fatores de Troca do Nucleotídeo Guanina
/
Vesículas Transportadoras
/
Proteínas de Ligação a DNA
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Eur J Cell Biol
Ano de publicação:
2007
Tipo de documento:
Article