Effect of fluoroquinolone exposure on the proteome of Salmonella enterica serovar Typhimurium.
J Antimicrob Chemother
; 58(6): 1145-53, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-17062612
ABSTRACT
OBJECTIVES:
The physiological response of Salmonella enterica serovar Typhimurium to fluoroquinolone antibiotics was investigated using proteomic methods.METHODS:
Proteomes were prepared from strain SL1344 following treatment of broth cultures with ciprofloxacin (0.03 and 0.008 mg/L; 2x and 0.5x MIC) and enrofloxacin (0.03 mg/L) and from a multiple antibiotic resistant (MAR) mutant. Protein expression was determined by two-dimensional HPLC-MS(n) and also after exposure to ciprofloxacin by two-dimensional gel electrophoresis (2D-GE).RESULTS:
The number of proteins (mean +/- SD) detected by 2D-GE derived from control cultures of the wild-type strain was significantly (P < 0.05) reduced from 296 +/- 77 to 153 +/- 36 following treatment with ciprofloxacin (0.03 mg/L). Raised expression (P < 0.05) of 17 proteins was also detected, and increases of up to 8-fold (P < 0.0001) were observed for subunits of F1F0-ATP synthase, TolC and Imp. Analysis by two-dimensional HPLC-MS(n) provided higher proteome coverage with 787 +/- 50 proteins detected, which was reduced (P < 0.005) to 560 +/- 14 by ciprofloxacin (0.03 mg/L). Increased expression of 43 proteins was observed which included those detected by 2D-GE and additionally the efflux pump protein AcrB. The basal expression of the AcrAB/TolC efflux pump was elevated in the MAR mutant compared with the untreated wild-type and augmented following treatment with ciprofloxacin (0.03 mg/L). F1F0-ATP synthase and Imp were only elevated in the mutant when treated with ciprofloxacin.CONCLUSIONS:
These studies suggest that increased expression of AcrAB/TolC was associated with resistance while other increases, such as in F1F0-ATP synthase and Imp, were a response to fluoroquinolone.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Salmonella typhimurium
/
Proteínas de Bactérias
/
Adaptação Fisiológica
/
Proteoma
/
Fluoroquinolonas
/
Antibacterianos
Idioma:
En
Revista:
J Antimicrob Chemother
Ano de publicação:
2006
Tipo de documento:
Article