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Characterization of the androgen receptor in a benign prostate tissue-derived human prostate epithelial cell line: RC-165N/human telomerase reverse transcriptase.
Kim, K-H; Dobi, A; Shaheduzzaman, S; Gao, C L; Masuda, K; Li, H; Drukier, A; Gu, Y; Srikantan, V; Rhim, J S; Srivastava, S.
Afiliação
  • Kim KH; Department of Surgery, Center for Prostate Disease Research, Uniformed Services University, Bethesda, MD 20852, USA.
Prostate Cancer Prostatic Dis ; 10(1): 30-8, 2007.
Article em En | MEDLINE | ID: mdl-17075604
The majority of prostate epithelial cell lines stably expressing wild-type (wt) or mutant (mt) androgen receptor (AR) are derived from metastatic prostate cancers. Therefore, the wt AR-expressing RC-165N/human telomerase reverse transcriptase (hTERT) cell line derived from the benign prostate tissue of an African-American patient provides a unique opportunity to assess the functional status of AR in a cellular context not studied before. Although androgen-induced expression of known androgen responsive genes such as PMEPA1, and NDRG1 was observed in RC-165N/hTERT, this cell line expresses prostate-specific antigen (PSA) at significantly lower levels. Chromatin immunoprecipitation assay revealed androgen-dependent binding of AR to androgen response elements of PSA, PMEPA1 and NDRG1 genes. Similarities, as well as differences were noted in the expression of androgen responsive genes between RC-165N/hTERT and LNCaP cells. Comprehensive evaluations of AR functions in RC-165N/hTERT cells suggest that whereas some features of known AR functions are maintained in this benign prostatic tissue-derived cell line, other AR functions are not retained. Objective evaluations of similar cell lines will lead to the understanding of AR functions in prostate growth and differentiation.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hiperplasia Prostática / Receptores Androgênicos / Telomerase / Células Epiteliais Limite: Humans / Male Idioma: En Revista: Prostate Cancer Prostatic Dis Ano de publicação: 2007 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hiperplasia Prostática / Receptores Androgênicos / Telomerase / Células Epiteliais Limite: Humans / Male Idioma: En Revista: Prostate Cancer Prostatic Dis Ano de publicação: 2007 Tipo de documento: Article