Differentiating effect of thalidomide and FGM-CS combination on HL-60 acute promyelocytic leukemia cells.
Exp Oncol
; 28(3): 216-9, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-17080015
ABSTRACT
AIM:
To investigate whether granulocyte-macrophage colony-stimulating factor (GM-CSF) with or without thalidomide can induce apoptosis and differentiation of HL-60 acute promyelocytic leukemia cell line in vitro.METHODS:
Effect of GM-CSF and thalidomide on proliferation of HL-60 cells was evaluated by MTT assay, cell cycle analysis was performed by propidium iodide staining approach and flow cytometry, and apoptosis rate was analyzed using FITC-conjugated annexin-V and FACScan flow cytometry.RESULTS:
The study revealed that thalidomide alone at high concentrations inhibited HL-60 cell growth and induced apoptosis. Three days treatment of low-dose thalidomide in combination with GM-CSF induced marked terminal differentiation of HL-60 cells, as it was assessed by increased expression of differentiation antigens on cell surface.CONCLUSION:
Treatment of HL-60 cells by low concentration of thalidomide combined with GM-CSF induced terminal differentiation of HL60 cells in vitro, which may be advantageous for the elaboration of novel therapeutic regimens in patients with differentiation-inducible leukemias.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Talidomida
/
Leucemia Promielocítica Aguda
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
Limite:
Humans
Idioma:
En
Revista:
Exp Oncol
Ano de publicação:
2006
Tipo de documento:
Article