Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine.
Forensic Sci Int
; 171(1): 44-51, 2007 Aug 24.
Article
em En
| MEDLINE
| ID: mdl-17097252
ABSTRACT
A fast method was designed for the simultaneous determination of amphetamine (A), methamphetamine (MA), PMA, MDA, MDMA, MDEA and MBDB in urine. The drugs were analysed by LC (ESI)-MS/MS, after a simple liquid-liquid extraction in the presence of the deuterated analogues. Reverse phase separation on an Atlantis dC18 Intelligent Speed column was achieved in less than 4 min under gradient conditions, and the total run time was 8 min. The method was fully validated, including linearity (1-1000 ng/mL for A, MDMA, MDEA and MBDB; 2-1000 ng/mL for MDA and PMA; 1-200 ng/mL for MA; r2>0.99 for all compounds), recovery (>80%), within-day and between-day precision and accuracy (CV and MRE<12.7% for intermediate level and ULOQ, and <17.2% for LLOQ), limit of detection (0.2 ng/mL for MDMA, MDEA and MBDB; 0.5 ng/mL for A, MA and PMA; 1 ng/mL for MDA) and quantitation (1 ng/mL for A, MA, MDMA, MDEA and MBDB; 2 ng/mL for MDA and PMA) and relative ion intensities. No matrix effect was observed. The procedure proved to be sensitive, specific and rapid, and was applied to real forensic cases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxicologia Forense
/
Alucinógenos
/
Anfetaminas
/
Cromatografia Gasosa-Espectrometria de Massas
Limite:
Adolescent
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Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Forensic Sci Int
Ano de publicação:
2007
Tipo de documento:
Article