High level expression of biologically active canine interferon-alpha subtype 4 using a baculovirus.
J Vet Med Sci
; 68(12): 1347-9, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-17213707
ABSTRACT
In this study, a high amount of bioactive recombinant canine interferon-alpha subtype 4 (CaIFN-alpha4) was expressed in a baculovirus system. For easy purification, it was expressed as a CaIFN-alpha4 bearing histidine hexamer at the C-terminal region, designated CaIFN-alpha4His. CaIFN-alpha4His was detected in culture supernatants of insect cells infected with the recombinant virus using sodium dodecyl sulfate-polyarcylamide gel electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue staining. The level of expression was very high, and approximately 1 mg of purified protein, with 5.0 x 10(7) units/mg, was obtained from 300 ml of culture supernatant. The purified product showed antiviral activity against Vesicular stomatitis virus on canine tumor cell line A72 and chicken embryo fibroblast cells.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interferon-alfa
/
Cães
Limite:
Animals
Idioma:
En
Revista:
J Vet Med Sci
Ano de publicação:
2006
Tipo de documento:
Article