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Effect of a bone marrow microenvironment on the ex-vivo expansion of umbilical cord blood progenitor cells.
Galan, I; DeLeon, J A; Diaz, L; Hong, J-S; Khalek, N; Munoz-Fernandez, M A; Santolaya-Forgas, J.
Afiliação
  • Galan I; The Women's Health Research Institute, Texas Tech University Health Science Center at Amarillo, Amarillo, TX, USA.
Int J Lab Hematol ; 29(1): 58-63, 2007 Feb.
Article em En | MEDLINE | ID: mdl-17224009
Progenitor cells (CD34(+)) can be isolated from umbilical cord blood and used to correct or reconstitute various cell lines within the haematopoietic and endothelial cell lineage. The main disadvantage of this procedure relates to the low volume of blood that can be collected after the umbilical cord has been clamped, which limits the number of progenitor cells available for treatment. This limitation, however, can be overcome by expanding CD34(+) cells ex vivo. Our aim was to perform a controlled study to determine if the ex-vivo proliferation of umbilical cord CD34(+) cells is enhanced when they are placed in a system that mimics the bone marrow microenvironment. For this purpose, CD34(+) cells were isolated from umbilical cord blood using a magnetic cell sorting kit and seeded in platforms containing different cocktails of cytokines with and without a three-dimensional (3D) biomatrix. Results from this study suggest that the number of viable cells can double after 1 week in any of the culture platforms and that the 3D biomatrix does not enhance cell proliferation.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Medula Óssea / Células-Tronco Hematopoéticas / Antígenos CD34 / Proliferação de Células / Sangue Fetal Limite: Humans Idioma: En Revista: Int J Lab Hematol Ano de publicação: 2007 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Medula Óssea / Células-Tronco Hematopoéticas / Antígenos CD34 / Proliferação de Células / Sangue Fetal Limite: Humans Idioma: En Revista: Int J Lab Hematol Ano de publicação: 2007 Tipo de documento: Article