Sample capacity in preparative high-speed counter-current chromatography.
J Chromatogr A
; 1146(2): 186-92, 2007 Apr 06.
Article
em En
| MEDLINE
| ID: mdl-17303145
ABSTRACT
High-speed counter-current chromatography (HSCCC) is a versatile technique in preparative separation and purification of pure compounds from complex matrices. As a preparative chromatography, there is a need to maximize the column production. Based on the plate theory of Van Deemter, the effect of the sample load on the separation was investigated in a preparative HSCCC with a 1000 ml column capacity. The test samples of hydroquinone, pyrocatechol and phenol were separated using a two-phase solvent system of n-hexane-ethyl acetate-ethanol-water (1111, v/v/v/v) at different sample loads. The results showed that for the case of HSCCC, the agreement of the effect of sample load on peak height and peak width between the Van Deemter's theory and the experiments is excellent. Furthermore, the factors limiting the mass load, including the resolution between the peaks, the partition isotherm and the solute solubility were also discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Distribuição Contracorrente
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2007
Tipo de documento:
Article