[HTLV-1 and HTLV-2 proviral load: a simple method using quantitative real-time PCR]. / Carga proviral do HTLV-1 e HTLV-2: um método simples através da PCR quantitativa em tempo real.
Rev Soc Bras Med Trop
; 39(6): 548-52, 2006.
Article
em Pt
| MEDLINE
| ID: mdl-17308700
ABSTRACT
When the human T cell lymphotropic virus (HTLV) is integrated with the host cell genome (provirus), its proviral DNA is a replication marker. Proviral load appears to be an important factor in the development of diseases related to these retroviruses. In this study, a methodology for absolute quantification of the HTLV-1 and HTLV-2 proviral load using real-time PCR was developed. Fifty-three blood donor samples with positive ELISA test result were subjected to this methodology, which utilized the TaqMan system for three target sequences HTLV-1, HTLV-2 and albumin. The absolute proviral load was quantified using the relative ratio between the HTLV genome and the host cell genome, taking into consideration the white blood cell count. The method presented is sensitive (215 copies/ml), practical and simple for proviral quantification, and is efficient and appropriate for confirming and discriminating infections according to viral type.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus Linfotrópico T Tipo 1 Humano
/
Vírus Linfotrópico T Tipo 2 Humano
/
Reação em Cadeia da Polimerase
/
Provírus
/
Carga Viral
Limite:
Adult
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
Pt
Revista:
Rev Soc Bras Med Trop
Ano de publicação:
2006
Tipo de documento:
Article