Substrate specificity of Escherichia coli thymidine phosphorylase.
Biochemistry (Mosc)
; 72(1): 21-8, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17309433
ABSTRACT
Substrate specificity of Escherichia coli thymidine phosphorylase to thymidine derivatives modified at 5' -, 3' -, and 2' ,3' - positions of the sugar moiety was studied. Equilibrium and kinetic constants (K(m), K(I), k(cat)) of the phosphorolysis reaction have been determined for 20 thymidine analogs. The results are compared with X-ray and molecular dynamics data. The most important hydrogen bonds in the enzyme-substrate complex are revealed.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Timidina
/
Timidina Fosforilase
/
Substituição de Aminoácidos
/
Escherichia coli
Idioma:
En
Revista:
Biochemistry (Mosc)
Ano de publicação:
2007
Tipo de documento:
Article