Your browser doesn't support javascript.
loading
Regioselective separation of isomeric triacylglycerols by reversed-phase high-performance liquid chromatography: stationary phase and mobile phase effects.
Momchilova, Svetlana; Itabashi, Yutaka; Nikolova-Damyanova, Boryana; Kuksis, Arnis.
Afiliação
  • Momchilova S; Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria.
J Sep Sci ; 29(17): 2578-83, 2006 Nov.
Article em En | MEDLINE | ID: mdl-17313097
Complete regioselective separation of five pairs of isomeric dipalmitoyl polyalkenoyl glycerols with two to six double bonds in the unsaturated acyl residues has been achieved by RP-HPLC on a single ODS column. Four ODS columns with stationary phases containing different percentages of free silanol groups have been tested. Binary mobile phases of ACN admixed with dichloromethane, tetrahydrofuran, 1-propanol, 2-propanol, ethanol, or acetone have been examined. The choice of modifier depended on the nature of the stationary phase. The more polar solvents were better suited for stationary phases with higher percentage of free silanol groups. Isomeric species were eluted according to chain length, number of double bonds, and the position of the unsaturated acyl chain in the glycerol molecule. Retention increases in the order 20:5 < 22:6 < 18:3 < 20:4 << 18:2. Within each isomeric pair, the species with unsaturated acyl chain occupying either the sn-1- or the 3-position were retained preferentially. Complete simultaneous regioselective separation of 10 isomeric triacylglycerols in a single isocratic run on a single ODS column was demonstrated.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triglicerídeos Idioma: En Revista: J Sep Sci Ano de publicação: 2006 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triglicerídeos Idioma: En Revista: J Sep Sci Ano de publicação: 2006 Tipo de documento: Article