Reporter proteins for in vivo fluorescence without oxygen.
Nat Biotechnol
; 25(4): 443-5, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17351616
ABSTRACT
Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real-time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue-light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Genes Reporter
/
Proteínas Luminescentes
Idioma:
En
Revista:
Nat Biotechnol
Ano de publicação:
2007
Tipo de documento:
Article