Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-gamma expression.
J Ethnopharmacol
; 112(2): 232-6, 2007 Jun 13.
Article
em En
| MEDLINE
| ID: mdl-17408892
ABSTRACT
Bidens pilosa is used as an ethnical medicine for bacterial infection or immune modulation in Asia, America and Africa. Here, we employed an IFN-gamma promoter-driven luciferase reporter construct and T cells to characterize immunomodulatory compounds from this plant based on a bioactivity-guided isolation principle. We found that PHA, a positive control, caused a six-fold increase in IFN-gamma promoter activity. In contrast, hot water crude extracts from Bidens pilosa and its butanol subfraction increased IFN-gamma promoter activity to two- and six-fold, respectively. Finally, centaurein (EC(50)=75 microg/ml) and its aglycone, centaureidin (EC(50)=0.9 microg/ml), isolated from this butanol subfraction, augmented IFN-gamma promoter activity by approximately four-fold. Consistent with the role of centaurein or its aglycone in IFN-gamma regulation, we showed that centaurein induced the activity of NFAT and NFkappaB enhancers, located within the IFN-gamma promoter, in Jurkat cells. Overall, our results showed that centaurein regulated IFN-gamma transcription, probably via NFAT and NFkappaB in T cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Flavonoides
/
Indutores de Interferon
/
Interferon gama
/
Bidens
/
Glucosídeos
Limite:
Humans
Idioma:
En
Revista:
J Ethnopharmacol
Ano de publicação:
2007
Tipo de documento:
Article