Bifunctional nanotube scaffolds for diverse ligands are purified simply from Escherichia coli strains coexpressing two functionalized flagellar genes.
Nano Lett
; 7(6): 1809-16, 2007 Jun.
Article
em En
| MEDLINE
| ID: mdl-17489638
ABSTRACT
We functionalized Escherichia coli FliC flagellin proteins to form tailored nanotubes binding single types or pairs of ligands, including divalent cations, fluorescent antibodies, or biotin-avidin-linked moieties such as ferritins. The ratio of each tag in bifunctionalized flagella could be toggled extending their sophistication as nanoscaffolds. Tobacco Etch Virus (TEV) protease site-containing FliCs were cleaved by the cognate protease without filament disintegration, potentiating their use as removable nanolithography masks to deposit attached ligands by protease cleavage.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Cristalização
/
Proteínas de Escherichia coli
/
Nanotecnologia
/
Nanoestruturas
/
Escherichia coli
Idioma:
En
Revista:
Nano Lett
Ano de publicação:
2007
Tipo de documento:
Article