An efficient chemoenzymatic production of small molecule glucosides with in situ UDP-glucose recycling.
FEBS Lett
; 581(13): 2562-6, 2007 May 29.
Article
em En
| MEDLINE
| ID: mdl-17499718
ABSTRACT
A one-pot system for efficient enzymatic synthesis of curcumin glucosides is described. The method couples the activities of two recombinant enzymes, UDP-glucose curcumin glucosyltransferase from Catharanthus roseus (CaUGT2) and sucrose synthase from Arabidopsis thaliana (AtSUS1). UDP, a product inhibitor of UDP-glucosyltransferase, was removed from the system and used for regeneration of UDP-glucose by the second enzyme, AtSUS1. The productivity was increased several-fold and UDP-glucose initially added to the reaction mixture could be reduced to one-tenth of the normal level. The concept of enhancing glucosylation efficiency by coupling a UDP-glucose regeneration system with glucosyltransferases should be applicable to enzymatic production of a wide range of glucosides.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Uridina Difosfato Glucose
/
Glucosídeos
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2007
Tipo de documento:
Article