Regional differences in expression of TPH-1, SERT, 5-HT(3) and 5-HT(4) receptors in the human stomach and duodenum.
Neurogastroenterol Motil
; 19(5): 342-8, 2007 May.
Article
em En
| MEDLINE
| ID: mdl-17509016
ABSTRACT
The aim of this study was to increase the understanding of the role of serotonergic signalling in normal gastroduodenal function at a molecular level. Mucosal biopsy specimens were collected from the fundus, antrum and duodenum of 11 healthy subjects. Serotonin (5-HT)-positive cells were counted and the mRNA levels of tryptophan hydroxylase (TPH), serotonin transporter (SERT), 5-HT(4) receptor and 5-HT(3) receptor subunits were quantified by real-time reverse transcription polymerase chain reaction. The number of 5-HT-positive cells was larger in the duodenum compared with the stomach (P < 0.001). Serotonin transport protein expression was 19-fold higher in the duodenum compared with the antrum and 457-fold higher compared with the fundus (P < 0.001). Tryptophan hydroxylase-1 expression was lower in the duodenum compared with the antrum and fundus (regional differences -2.3 and -3.6, respectively). The 5-HT(4) receptor and the 5-HT(3C) and 5-HT(3E) receptor subunits were more abundantly expressed in duodenum compared with the stomach (P < 0.001). The larger number of 5-HT-positive cells, the higher expression of 5-HT(3) and 5-HT(4) receptors, and in particularly the higher uptake capacity of 5-HT in the duodenum, point out to a more prominent role of serotonergic signalling at the mucosal level in the duodenum compared with the stomach.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triptofano Hidroxilase
/
Receptores 5-HT4 de Serotonina
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Receptores 5-HT3 de Serotonina
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Duodeno
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Proteínas da Membrana Plasmática de Transporte de Serotonina
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Mucosa Gástrica
Limite:
Adult
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Female
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Humans
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Middle aged
Idioma:
En
Revista:
Neurogastroenterol Motil
Ano de publicação:
2007
Tipo de documento:
Article