Proteomic analysis to dissect mitoxantrone resistance-associated proteins in a squamous lung carcinoma.
Anticancer Res
; 27(3A): 1277-84, 2007.
Article
em En
| MEDLINE
| ID: mdl-17593620
ABSTRACT
BACKGROUND:
Mitoxantrone resistance has been related to the expression of a drug efflux pump breast cancer resistance pump (BCRP) but little is known of the intracellular protein changes. In this work, differential protein expression in a squamous lung carcinoma cell line, DLKP, and its mitoxantrone-resistant variant (DLKP-Mitox) was investigated to elucidate other changes associated with mitoxantrone resistance. MATERIALS ANDMETHODS:
Differential protein expression between DLKP and DLKP-Mitox was investigated using 2D-DIGE technology. Proteins of interest were identified by MALDI-ToF mass spectrometry. Western blotting was used to confirm and validate some of these changes.RESULTS:
Biological variation analysis in Decyder software revealed a total of 343 proteins to be differentially regulated with p < 0.05. Identification of 61 proteins of interest by mass spectrometry revealed changes in proteins involved in many cellular processes including apoptosis and differentiation.CONCLUSION:
Alterations in these cellular processes and proteins present alternative sites to circumvent resistance to mitoxantrone.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carcinoma de Células Escamosas
/
Mitoxantrona
/
Neoplasias Pulmonares
/
Antineoplásicos
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2007
Tipo de documento:
Article