Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses.
Appl Environ Microbiol
; 73(17): 5653-6, 2007 Sep.
Article
em En
| MEDLINE
| ID: mdl-17601805
ABSTRACT
Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bifidobacterium
/
Proteômica
/
Marcação por Isótopo
/
Leucina
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2007
Tipo de documento:
Article