Platelet aggregation is affected by nitrosothiols in patients with chronic hepatitis: in vivo and in vitro studies.
World J Gastroenterol
; 13(27): 3677-83, 2007 Jul 21.
Article
em En
| MEDLINE
| ID: mdl-17659726
ABSTRACT
AIM:
To investigate the relationship among the number of platelets and plasma levels of S-nitrosothiols (S-NO), nitrite, total non-protein SH (NPSH), glutathione (GSH), cysteine (CYS), malondialdehyde (MDA), 4-hydroxininenal (4HNE), tumor necrosis factor-alpha (TNFalpha) and interleukin (IL)-6 in patients with chronic hepatitis C (CH).METHODS:
In vitro the aggregation of platelets derived from controls and CH patients was evaluated before and after the addition of adenosine diphosphate (ADP) and collagen, both in basal conditions and after incubation with nitrosoglutathione (GSNO).RESULTS:
In vivo, S-NO plasma levels increased significantly in CH patients and they were significantly directly correlated with platelet numbers. Patients with platelet counts < 150000/microL, had a smaller increase in S-NO, lower levels of GSH, CYS, NPSH, TNFalpha, and IL-6, and higher levels of nitrite, MDA, and 4-HNE relative to those of patients with platelet counts > 150000/microL. In vitro, the ADP and collagen aggregation time was increased in platelets from patients and not from controls; in addition, platelets from CH patients but not from controls also showed a latency time after exposure to collagen.CONCLUSION:
The incubation of platelets with GSNO improved the percentage aggregation and abolished the latency time.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Trombocitopenia
/
Plaquetas
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Agregação Plaquetária
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Hepatite C Crônica
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S-Nitrosotióis
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Óxido Nítrico
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Adult
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Aged
/
Aged80
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
World J Gastroenterol
Ano de publicação:
2007
Tipo de documento:
Article