Lipophosphoramidates as lipidic part of lipospermines for gene delivery.
Bioconjug Chem
; 18(5): 1575-82, 2007.
Article
em En
| MEDLINE
| ID: mdl-17676781
ABSTRACT
The DNA compacting properties of polyamines (especially spermine) are well-known, hence the use of spermine as the cationic part in several synthetic DNA carriers. Here, we describe the synthesis of modified spermines, with a "lipophosphoramidate" as the lipidic part, and their use for efficient in vitro transfection. Physicochemical measurements (particle size, zeta potentials, pKa determination) and gel retardation assays were also performed. Theoretical membrane-disrupting ability was established by FRET. Taken together, our results indicate that lipophosphoramidates constitute an interesting alternative to "classical" lipidic parts of cationic lipids used as DNA carriers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidiletanolaminas
/
DNA
/
Portadores de Fármacos
/
Espermina
/
Técnicas de Transferência de Genes
/
Nanoestruturas
/
Lipossomos
Idioma:
En
Revista:
Bioconjug Chem
Ano de publicação:
2007
Tipo de documento:
Article