Gonococcal transferrin binding protein chimeras induce bactericidal and growth inhibitory antibodies in mice.
Vaccine
; 25(41): 7247-60, 2007 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-17720283
ABSTRACT
We have previously demonstrated the full-length gonococcal transferrin binding proteins (TbpA and TbpB) to be promising antigens in the development of a protective vaccine against Neisseria gonorrhoeae. In the current study we employed a genetic chimera approach fusing domains from TbpA and TbpB to the A2 domain of cholera toxin, which naturally binds in a non-covalent fashion to the B subunit of cholera toxin during assembly. For one construct, the N-terminal half of TbpB (NB) was fused to the A2 subunit of cholera toxin. In a second construct, the loop 2 region (L2) of TbpA was genetically fused between the NB domain and the A2 domain, generating a double chimera. Both chimeras were immunogenic and induced serum bactericidal and vaginal growth-inhibiting antibodies. This study highlights the potential of using protective epitopes instead of full-length proteins in the development of an efficacious gonococcal vaccine.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Gonorreia
/
Proteína A de Ligação a Transferrina
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Proteína B de Ligação a Transferrina
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Viabilidade Microbiana
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Anticorpos Antibacterianos
/
Neisseria gonorrhoeae
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2007
Tipo de documento:
Article