High level production of Escherichia coli outer membrane proteins OmpA and OmpF intracellularly in Bacillus subtilis.
FEMS Microbiol Lett
; 67(1): 29-33, 1991 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-1778419
ABSTRACT
A high yield of Escherichia coli outer membrane proteins OmpA (about 200 mg/l) and OmpF (about 100 mg/l) was obtained in Bacillus subtilis when produced intracellularly. The yield was more than 100-fold higher than the yield of these proteins by a similar vector containing the complete signal sequence of alpha-amylase of B. amyloliquefaciens. Both proteins isolated after breakage of the B. subtilis cells by low-speed centrifugation were about 70% pure and could be solubilized by Sarkosyl, SDS and guanidine hydrochloride.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Proteínas da Membrana Bacteriana Externa
/
Escherichia coli
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1991
Tipo de documento:
Article