Site-specific incorporation of keto amino acids into functional G protein-coupled receptors using unnatural amino acid mutagenesis.

Ye, Shixin; Köhrer, Caroline; Huber, Thomas; Kazmi, Manija; Sachdev, Pallavi; Yan, Elsa C Y; Bhagat, Aditi; RajBhandary, Uttam L; Sakmar, Thomas P

Ye, Shixin; Köhrer, Caroline; Huber, Thomas; Kazmi, Manija; Sachdev, Pallavi; Yan, Elsa C Y; Bhagat, Aditi; RajBhandary, Uttam L; Sakmar, Thomas P.

Afiliação

Ye S; Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065.
Köhrer C; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
Huber T; Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065.
Kazmi M; Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065.
Sachdev P; Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065.
Yan ECY; Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065.
Bhagat A; Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065.
RajBhandary UL; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139. Electronic address: bhandary@mit.edu.
Sakmar TP; Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10065. Electronic address: sakmar@rockefeller.edu.

J Biol Chem ; 283(3): 1525-1533, 2008 Jan 18.

Article em En | MEDLINE | ID: mdl-17993461

Assuntos

Aminoácidos/metabolismo; Mutagênese Sítio-Dirigida; Receptores Acoplados a Proteínas G/metabolismo; Aminoacilação; Benzofenonas/metabolismo; Linhagem Celular; Escherichia coli/enzimologia; Geobacillus stearothermophilus/metabolismo; Humanos; Luciferases/metabolismo; Proteínas Mutantes/metabolismo; Mutação/genética; Fenilalanina/análogos & derivados; Fenilalanina/metabolismo; RNA de Transferência de Tirosina/metabolismo; Receptores CCR5/metabolismo; Rodopsina/metabolismo; Tirosina-tRNA Ligase/metabolismo

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Mutagênese Sítio-Dirigida / Receptores Acoplados a Proteínas G / Aminoácidos Limite: Humans Idioma: En Revista: J Biol Chem Ano de publicação: 2008 Tipo de documento: Article

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