Coated endosomal vesicles: sorting and recycling compartment for transferrin in BHK cells.
Eur J Cell Biol
; 56(2): 210-22, 1991 Dec.
Article
em En
| MEDLINE
| ID: mdl-1802708
ABSTRACT
We have investigated receptor-mediated endocytosis of transferrin (Tf) in baby hamster kidney (BHK) cells, using fluorescence and electron microscopy, and by carrying out colocalization experiments with clathrin antibodies and a fluorescently tagged glycolipid. Early during internalization, Tf was found in small vesicles (100-150 nm in diameter) located at the cell periphery. The ligand remained associated with such vesicles when the latter concentrated towards the cell center, before ending up in the juxtanuclear area. Throughout this vesicular trafficking pathway, clathrin colocalized with Tf. We conclude that Tf is processed intracellularly via small coated endosomal vesicles (CEV) and is not delivered into large tubular endosomes (CURL; compartment for uncoupling receptors and ligands), typical for ligand trafficking to lysosomes. By determining the kinetics of Tf internalization and by comparing the flow of Tf to that of a fluorescent glycolipid, it can also be concluded that CEVs display sorting and recycling properties, implying that small vesicles can be shed from or fuse with CEVs. Acidic pH does not prevent the formation of CEVs, but their intracellular movement, towards the cell center, is impeded.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacúolos
/
Transferrina
/
Invaginações Revestidas da Membrana Celular
/
Endocitose
Limite:
Animals
Idioma:
En
Revista:
Eur J Cell Biol
Ano de publicação:
1991
Tipo de documento:
Article