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Computational prediction and identification of dengue virus-specific CD4(+) T-cell epitopes.
Wen, Jin-Sheng; Jiang, Li-Fang; Zhou, Jun-Mei; Yan, Hui-Jun; Fang, Dan-Yun.
Afiliação
  • Wen JS; Department of Microbiology, Zhongshan Medical College, Sun Yat-Sen University, Guangzhou 510080, People's Republic of China.
Virus Res ; 132(1-2): 42-8, 2008 Mar.
Article em En | MEDLINE | ID: mdl-18061300
In this study, we tried to identify dengue virus-specific CD4(+) T-cell epitopes, which can induce PBMC (peripheral blood mononuclear cells) isolated from DF convalescent patients (dengue virus type 1 infection) to secrete IFN-gamma. PBMC of DF convalescent patients were stimulated in vitro with dengue virus-derived peptides, which were prepared based on the prediction of dengue virus-specific CD4(+) T-cell epitopes by using RANKpep online software. Subsequently, the frequency of IFN-gamma producing T cells and percentage of IFN-gamma(+) CD4(+) T cells were measured by using ELISPOT assay and ICS assay (intracellular cytokine straining), respectively. The positive response of PBMC by ELISPOT showed that the numbers of SFC (spots forming cells) ranged from 50 to 310 SFC/1x10(6) PBMC. The positive response of PBMC by ICS assay showed that the percentage of IFN-gamma(+) CD4(+) T cells ranged from 0.03 to 0.27%. As a result, C(45-57) (KLVMAFIAFLRFL), E(396-408) (SSIGKMFEATARG), NS3(23-35) (YRILQRGLLGRSQ), and NS3(141-155) (NREGKIVGLYGNGVV) were identified as dengue virus-specific CD4(+) T-cell epitopes.
Assuntos

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Peptídeos / Linfócitos T CD4-Positivos / Epitopos de Linfócito T / Vírus da Dengue / Antígenos Virais Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Virus Res Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Peptídeos / Linfócitos T CD4-Positivos / Epitopos de Linfócito T / Vírus da Dengue / Antígenos Virais Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limite: Adolescent / Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Virus Res Ano de publicação: 2008 Tipo de documento: Article