Extract of Ginkgo biloba induces glutamate cysteine ligase catalytic subunit (GCLC).
Phytother Res
; 22(3): 367-71, 2008 Mar.
Article
em En
| MEDLINE
| ID: mdl-18167050
ABSTRACT
The extract of Ginkgo biloba (EGb), containing 24% flavone glycosides and 6% terpenoids, is widely used to treat early-stage Alzheimer's disease, vascular dementia, peripheral claudication and vascular tinnitus. Its marked antioxidant activity has recently been demonstrated in both cell lines and animals. Glutathione (GSH) plays an important role in the antioxidant system by conjugating to xenobiotics to facilitate their export from cells. Glutamate cysteine ligase (GCL) is the rate-limiting enzyme for GSH synthesis and its catalytic subunit (GCLC) determines this de novo synthesis. Thus, induction of GCLC is a strategy to enhance the antioxidant capability in cells. The present study aimed to investigate the induction effect of EGb on GCLC in HepG2 and Hep1c1c7 cell lines. Real-time PCR, Western blot and enzyme activity assay were used to detect induction and it was found that GCLC was induced by EGb in these two cell lines. It is suggested that the antioxidant activity of EGb is (or is partly) through the induction of GCLC.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Regulação da Expressão Gênica de Plantas
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Ginkgo biloba
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Domínio Catalítico
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Glutamato-Cisteína Ligase
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Phytother Res
Ano de publicação:
2008
Tipo de documento:
Article