Adult olfactory bulbs from primates provide reliable ensheathing glia for cell therapy.
Glia
; 56(5): 539-51, 2008 Apr.
Article
em En
| MEDLINE
| ID: mdl-18240311
ABSTRACT
Olfactory bulb ensheathing glia (OB-OEG) from adult rodents promote functional and morphological repair after grafting into injured spinal cords. To provide insight into the feasibility of using OB-OEG in human therapy, we studied OB-OEG in primates to determine their suitability for spinal cord transplantation. Here, we show that OEG can be obtained from olfactory bulbs of adult macaca mulatta and nemestrina monkeys and compare their characteristics to those obtained from rats. In contrast to rodent OB-OEG, primate OB-OEG are nonsenescent, exhibit a longer lifespan, are less sensitive to high oxygen culture environment, and maintain a phenotype suitable for grafting for up to 2.5 months in vitro. Three-week cultures (short term) derived from a single macaca olfactory bulb provide enough OEG for autologous transplantation at the acute stage of injury, and after long-term cultures (2.5 months) may yield an additional 20 billion. OEG can be frozen for later use. Therefore, primate adult olfactory bulbs constitute a reliable source of OEG for cell therapy, and successful culture of these cells make autologous transplantation feasible.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bulbo Olfatório
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Primatas
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Células Cultivadas
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Neuroglia
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Terapia Baseada em Transplante de Células e Tecidos
Limite:
Animals
Idioma:
En
Revista:
Glia
Ano de publicação:
2008
Tipo de documento:
Article