Mapping IgE-binding epitopes of Ric c 1 and Ric c 3, allergens from Ricinus communis, by mast cell degranulation assay.
Peptides
; 29(4): 497-504, 2008 Apr.
Article
em En
| MEDLINE
| ID: mdl-18262682
ABSTRACT
Ric c 1 and Ric c 3 are the major castor bean allergens. In order to identify continuous IgE-epitopes in Ric c 1 and Ric c 3, pools of sera from rats immunized with a pool of 2S albumin from these seeds, Ric c 1 and Ric c 3 overlapping synthetic peptides, were used to screen for IgE-binding epitopes. The allergenic properties were monitored by mast cell degranulation assays, histamine quantification and human-IgE binding. Large and small chains isolated from these proteins present allergenic properties. Four continuous epitopes were identified in Ric c 3 and two in Ric c 1. This knowledge may allow the induction of protective antibody responses to antagonize the IgE recognition.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Ricinus
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Imunoglobulina E
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Antígenos de Plantas
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Mastócitos
/
Epitopos
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Peptides
Ano de publicação:
2008
Tipo de documento:
Article