Overview of the baculovirus expression system.
Curr Protoc Mol Biol
; Chapter 16: Unit 16.9, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-18265342
ABSTRACT
Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This unit gives an overview of the baculovirus expression system, including discussion of the baculovirus life cycle, and post-translational modifications that occur in insect cells. In addition, the steps for overproducing proteins in the baculovirus systems are described along with recommendations for choosing an appropriate baculovirus vector and DNA, and reagents and equipment necessary for implementing the whole overexpression system.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Baculoviridae
/
Clonagem Molecular
/
Vetores Genéticos
Limite:
Animals
Idioma:
En
Revista:
Curr Protoc Mol Biol
Ano de publicação:
2004
Tipo de documento:
Article