Trigeminal nasal-specific neurons respond to nerve growth factor with substance-P biosynthesis.
Clin Exp Allergy
; 38(7): 1203-11, 2008 Jul.
Article
em En
| MEDLINE
| ID: mdl-18307524
ABSTRACT
BACKGROUND:
Nerve growth factor (NGF) has been found to induce substance-P biosynthesis in large-diameter A-fibres vagal airway neurons. However, the effect of NGF on trigeminal neurons innervating the nasal mucosa of the mouse has not been investigated so far.OBJECTIVE:
NGF has been implicated in allergic diseases by modulating sensory nerves. Therefore, the present study investigated the effect of NGF on neuropeptides expression such as substance-P and glutamate in nasal trigeminal neurons.METHODS:
Using neuronal tracing in combination with double labelling immunohistochemistry the expression of substance-P, glutamate and neurofilament protein 68-kDa expression was examined in nasal-specific trigeminal neurons of BALB/c-mice.RESULTS:
The numbers of Fast blue-labelled trigeminal neurons expressing substance-P were significantly increased after NGF exposure (NGF-treated ganglia 16.4 +/- 0.6% vs. control 7.0 +/- 0.4%, PCONCLUSION:
Under normal conditions, substance-P was expressed in nasal-specific neurofilament-negative, glutamatergic and C-fibre neurons. Nasal-specific trigeminal neurons respond to NGF treatment with substance-P biosynthesis in non-glutamatergic, neurofilament-positive as well as -negative neurons. These findings suggest that nasal-specific trigeminal neurons are composed of heterogenous subpopulations in relation to their peptide profiles and therefore may have different functions in neurogenic airway inflammation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nervo Trigêmeo
/
Substância P
/
Fator de Crescimento Neural
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Mucosa Nasal
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Neurônios
Limite:
Animals
Idioma:
En
Revista:
Clin Exp Allergy
Ano de publicação:
2008
Tipo de documento:
Article