Image acquisition for colocalization using optical microscopy.
Am J Physiol Cell Physiol
; 294(5): C1119-22, 2008 May.
Article
em En
| MEDLINE
| ID: mdl-18353895
ABSTRACT
Colocalization, in which images of two or more fluorescent markers are overlaid, and coincidence between the probes is measured or displayed, is a common analytical tool in cell biology. Interpreting the images and the meaning of this identified coincidence is difficult in the absence of basic information about the acquisition parameters. In this commentary, we highlight important factors in the acquisition of images used to demonstrate colocalization, and we discuss the minimum information that authors should include in a manuscript so that a reader can interpret both the fluorescent images and any observed colocalization.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microscopia
/
Microscopia de Fluorescência
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Am J Physiol Cell Physiol
Ano de publicação:
2008
Tipo de documento:
Article