A subset of equine sarcoids harbours BPV-1 DNA in a complex with L1 major capsid protein.
Virology
; 375(2): 433-41, 2008 Jun 05.
Article
em En
| MEDLINE
| ID: mdl-18395238
ABSTRACT
Bovine papillomavirus type 1 or 2 (BPV-1, BPV-2) are accepted causal factors in equine sarcoid pathogenesis. Whereas viral genomes are consistently found and expressed within lesions, intact virions have never been detected, thus permissiveness of sarcoids for BPV-1 replication remains unclear. To reassess this issue, an immunocapture PCR (IC/PCR) was established using L1-specific antibodies to capture L1-DNA complexes followed by amplification of the viral genome. Following validation of the assay, 13 sarcoid-bearing horses were evaluated by IC/PCR. Samples were derived from 21 tumours, 4 perilesional/intact skin biopsies, and 1 serum. Tissue extracts from sarcoid-free equines served as controls. IC/PCR scored positive in 14/24 (58.3%) specimens obtained from sarcoid-patients, but negative for controls. Quantitative IC/PCR demonstrated <125 immunoprecipitable viral genomes/50 microl extract for the majority of specimens. Moreover, full-length BPV-1 genomes were detected in a complex with L1 proteins. These complexes may correspond to virion precursors or intact virions.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Sarcoidose
/
Neoplasias Cutâneas
/
Vírion
/
Infecções por Papillomavirus
/
Proteínas do Capsídeo
/
Papillomavirus Bovino 1
/
Doenças dos Cavalos
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
2008
Tipo de documento:
Article