Manganese-enhanced MRI detection of neurodegeneration in neonatal hypoxic-ischemic cerebral injury.
Magn Reson Med
; 59(6): 1329-39, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-18421694
ABSTRACT
In this study, we investigated the Mn-enhanced MRI (MEMRI) for detecting neurodegenerative processes in neonatal hypoxic-ischemic (H-I) cerebral injury. Seven-day-old rats were induced with H-I injury, and scanned for T1-weighted image (T1WI) and T2-weighted image (T2WI) with and without systemic MnCl2 administration. Serial histological analysis was performed for Mn-superoxide dismutase (Mn-SOD) and glutamine synthetase (GS), which are Mn-binding enzymes against the oxidative stress and glutamate excitotoxicity in neurodegeneration. In the acute phase (first 2 days), the ipsilateral lesion exhibited no Mn enhancement in T1WIs, with histology showing no Mn-SOD and GS production. In the mid-phase (from day 3), Mn enhancement was found in the cortex, basal ganglia, and hippocampus, correlating with local Mn-SOD and GS increase. In the late phase, the enhancement became more localized to the pericyst basal ganglia and cortex, and then gradually diminished. In T2WIs, a signal decrease was observed from day 3 in the corresponding regions. Hypointense voids gradually formed in the late phase, correlating with the local iron accumulation. H-I rats without Mn2+ administration exhibited similar but weak changes in T1WIs and T2WIs from days 14 and 7, respectively. These results indicate that Mn2+ may be a useful in vivo probe for monitoring Mn-SOD and GS enzymatic activities.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imageamento por Ressonância Magnética
/
Cloretos
/
Compostos de Manganês
/
Hipóxia-Isquemia Encefálica
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Magn Reson Med
Ano de publicação:
2008
Tipo de documento:
Article